Oligo Calculator

Dinucleotide thermodynamic parameters are used to calculate Tm.

                               deltaH
                Tm= -------------------------------  - 273.15 + 16.6log[Na+]
                      A + deltaS + Rln([oligo]/4)
A = -10.8 cal/(mol K)
R = 1.987 cal/(mol K) Gas constant
[oligo] = 5E-07 M
[Na+] = 0.05 M
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Enter Oligo Sequence in Box
Length Melting Temperature (Tm) °C
%GC content Molecular Weight: daltons (g/M)
OD of 1 is equal to picoMolar.